Anti-CCSP-Rabbit (WRAB-3950)使用說明
產(chǎn)品信息
品 牌 | Seven Hills Bioreagents |
貨 號(hào) | WRAB-3950 |
規(guī) 格 | 100 µL |
宿 主 | 兔 |
反應(yīng)種屬 | 人、鼠 |
克隆類型 | 多克隆 |
偶 聯(lián) 物 | 無 |
緩 沖 液 | 0.01% sodium azide |
抗 原 | 重組鼠CCSP融合6*his蛋白 |
保存條件 | 儲(chǔ)存在-20℃,避免反復(fù)凍融 |
蛋白背景 | Clara細(xì)胞分泌蛋白(CCSP)是Clara細(xì)胞最主要的分泌物,由二條相同肽鏈反向排列連接而成,CCSP作為肺上皮特異性蛋白已成為肺上皮屏障損傷的外周標(biāo)志。CCSP具有抗炎、抗氧化、免疫調(diào)節(jié)、腫瘤抑制等多種生物活性。 |
產(chǎn)品使用
實(shí)驗(yàn)應(yīng)用 | 建議稀釋倍數(shù) |
Western Blot | 1:10000 |
Immunohistochemistry | 1:20000 |
說明:最佳稀釋倍數(shù)與濃度應(yīng)由研究人員確認(rèn)
應(yīng)用實(shí)例
圖片為用抗CCSP兔血清(1:20000稀釋液)染色的成年小鼠肺組織。CCSP通過DAB(黑色)進(jìn)行可視化,并用Nuclear Fast Red(粉紅色)進(jìn)行復(fù)染。放大倍數(shù)20倍。
參考文獻(xiàn)
1. Harrod, KS, Mounday, AD, Stripp, BR, Whitsett, JA. 'Clara cell secretory protein decreases lung inflammation after acute virus infection.' Am J Physiol. 1998 Nov;275(5 Pt 1):L924-30.
2. Stripp BR, Lund J, Mango GW, Doyen KC, Johnston C, Hultenby K, Nord M, Whitsett JA. 'Clara cell secretory protein: a determinant of PCB bioaccumulation in mammals.' Am J Physiol. 1996 Oct;271(4 Pt 1):L656-64.
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