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Anti-Pro-SP-C - Rabbit, n-terminal (WRAB-9337)使用說(shuō)明

更新時(shí)間:2023-11-24      點(diǎn)擊次數(shù):913

Anti-Pro-SP-C - Rabbit, n-terminal (WRAB-9337)使用說(shuō)明

logo.jpg

產(chǎn)品信息

Seven Hills Bioreagents

號(hào)

WRAB-9337

規(guī)

100 µL

宿

反應(yīng)種屬

人、鼠

克隆類型

多克隆

聯(lián)

無(wú)

0.01% sodium azide

重組鼠肺表面活性蛋白C前體pro-SP-C, n-terminal (aa1-35) GST融合蛋白

保存條件

儲(chǔ)存在-20,避免反復(fù)凍融

產(chǎn)品使用

實(shí)驗(yàn)應(yīng)用

建議稀釋倍數(shù)

Western Blot

1:5000

Immunohistochemistry

1:3000

說(shuō)明:最佳稀釋倍數(shù)與濃度應(yīng)由研究人員確認(rèn)

應(yīng)用實(shí)例

圖片3.jpg

圖片成年小鼠肺組織用抗N-末端proSP-C兔血清(1:3000稀釋)染色。ProSP-C通過(guò)DAB(黑色)進(jìn)行可視化,并用Nuclear Fast Red(粉紅色)進(jìn)行復(fù)染。放大倍數(shù)40倍。

參考文獻(xiàn)

1. Glasser SW, Detmer EA, Ikegami M, Na CL, Stahlman MT, Whitsett JA. 'Pneumonitis and emphysema in sp-C gene targeted mice.' J Biol Chem. 2003 Apr 18;278(16):14291-8.

2. Nogee LM, Dunbar AE 3rd, Wert SE, Askin F, Hamvas A, Whitsett JA. 'A mutation in the surfactant protein C gene associated with familial interstitial lung disease.' N Engl J Med. 2001 Feb 22;344(8):573-9.

3. No abstract available. Glasser SW, Burhans MS, Korfhagen TR, Na CL, Sly PD, Ross GF, Ikegami M, Whitsett JA. 'Altered stability of pulmonary surfactant in SP-C-deficient mice.' Proc Natl Acad Sci U S A. 2001 May 22;98(11):6366-71.

4. Zhou L, Lim L, Costa RH, Whitsett JA. 'Thyroid transcription factor-1, hepatocyte nuclear factor-3beta, surfactant protein B, C, and Clara cell secretory protein in developing mouse lung.' J Histochem Cytochem. 1996 Oct;44(10):1183-93.

5. Yang, L., J. Johansson, R. Ridsdale, H. Willander, M. Fitzen, H. T. Akinbi, and T. E. Weaver. Surfactant Protein B Propeptide Contains a Saposin-Like Protein Domain with Antimicrobial Activity at Low pH. J Immunol 2010;184:975-983.

6. Conkright, J. J., K. S. Apsley, E. P. Martin, R. Ridsdale, W. R. Rice, C. L. Na, B. Yang, and T. E. Weaver. Nedd4-2-mediated ubiquitination facilitates processing of surfactant protein-C. Am J Respir Cell Mol Biol 2010;42:181-189.

7. Wang, M., J. P. Bridges, C. L. Na, Y. Xu, and T. E. Weaver. Meckel-Gruber syndrome protein MKS3 is required for endoplasmic reticulum-associated degradation of surfactant protein C. J Biol Chem 2009;284:33377-33383.

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