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EMFRET R300 產(chǎn)品說明書

更新時間：2017-07-05 點(diǎn)擊次數(shù)：5952

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德國EMFRET公司專注心血管和血液系統(tǒng)研究用抗體研發(fā)和生產(chǎn)，供應(yīng)的抗體包括清除血液中血小板、流式細(xì)胞術(shù)鑒定分析小鼠血小板表面糖蛋白，EMFRET公司的抗體不僅適用于流失分析、WB檢測，還可用于免疫組化/免疫熒光及免疫共沉淀檢測。

	Antibodies for Plaet Depletion in Mice
# R300
Product Information
Catalog Number:	R300 (lot # M)
Isotype:	Rat IgG (Wistar)
Contents:	0.5 mg purified immunoglobulins in PBS, sterile filtered
Concentration:	0.5 mg/ml

For research use only, not for diagnostic or therapeutic use. This product is no medical device.

Specificity: This antibody preparation contains a mixture of purified rat monoclonal antibodies

directed against mouse GPIba (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent plaet depletion in mice^1,2. Plaet depletion is frequently used to study the general role of plaets in (patho-) physiology³.

Preparation and Storage: The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography and contain < 0.00025 ng/µg (< 0.0025 EU/µg) protein endotoxin. Stable for six months from date of shipment when stored at 4°C. KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.

Usage: This preparation is optimized to reduce the plaet count by >95% within 60 minutes after intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 µl (diluted with sterile PBS).

Depletion of mouse plaets by anti-GPIba treatment

Wild type mice received 2 or 4 µg/g plaet depletion antibodies in 200 µl sterile PBS intravenously and plaet counts were determined at the indicated time points. Results are mean ± s.d. of 6 mice per group.

Nieswandt B, Bergmeier W, Rackebrandt K, Gessner JE, and Zirngibl H. (2000) Identification of critical antigen-specific mechanisms in the development of immune thrombocytopenic purpura in mice. Blood. 96:2520-27.
Elzey BD, Tian J, Jensen RJ et al. (2003) Plaet-mediated modulation of adaptive immunity. A communication link between innate and adaptive immune compartments. Immunity.19:9-19.

R300

Antibodies for Mouse Plaet Depletion

polyclonal anti-GPIb alpha

Rat IgG

purif.

plaet depletion

0.5 mg

其它產(chǎn)品信息如下：

貨號	抗原類型	克隆方式	表型	產(chǎn)品形式	應(yīng)用	規(guī)格
C301	清除小鼠血小板	polyclonal non-immune rat immunoglobulins	Rat IgG	純化	清除小鼠血小板	0.5 mg
D200	雙色分析血小板活性	Wug.E9 JON/A	Rat IgG2b/IgG1	FITC、PE	流式細(xì)胞術(shù)	2 x 1.0 ml; 200 tests
M011-0	GPVI	JAQ1	Rat IgG2a	純化	IP, IHC, WB	0.1 mg
M011-1	GPVI	JAQ1	Rat IgG2a	FITC	FC, IHC	1.5 ml; 300 tests
M021-0	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	Leo.H4	Rat IgG2b	purif.	IP, IHC, block	0.5 mg
M021-1	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	Leo.H4	Rat IgG2b	FITC	FC, IHC	1.5 ml; 300 tests
M022-0	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	Leo.A1	Rat IgG1	purif.	IP, IHC	0.5 mg
M023-2	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	JON/A	Rat IgG2b	PE	FC (activ. form)	1.5 ml; 300 tests
M024-0	Integrin alphaIIb (GPIIb, CD41)	MwReg30	Rat IgG1	purif.	IP, IHC, generation of Fab fragments	6 mg
M025-0	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	Leo.F2	Rat IgG2a	purif.	IP, IHC	0.5 mg
M025-1	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	Leo.F2	Rat IgG2a	FITC	FC, IHC	1.5 ml; 300 tests
M025-2	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	Leo.F2	Rat IgG2a	PE	FC, IHC	1.5 ml; 300 tests
M025-3	Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61)	Leo.F2	Rat IgG2a	DyLight 649	FC, IHC	1.5 ml; 300 tests
M030-0	Integrin beta3 chain (GPIIIa, CD61)	Luc.A5	Rat IgG2a	purif.	IP, IHC, WB	0.5 mg
M031-0	Integrin beta3 chain (GPIIIa, CD61)	Luc.H11	Rat IgG2b	purif.	IP, IHC, WB	0.5 mg
M031-1	Integrin beta3 chain (GPIIIa, CD61)	Luc.H11	Rat IgG2b	FITC	FC, IHC	1.5 ml; 300 tests
M040-0	GPIbalpha (CD42b)	Xia.G5	Rat IgG2b	purif.	IP, IHC	0.5 mg
M040-1	GPIbalpha (CD42b)	Xia.G5	Rat IgG2b	FITC	FC, IHC	1.5 ml; 300 tests
M040-2	GPIbalpha (CD42b)	Xia.G5	Rat IgG2b	PE	FC, IHC	1.5 ml; 300 tests
M040-3	GPIbalpha (CD42b)	Xia.G5	Rat IgG2b	DyLight 649	FC, IHC	1.5 ml; 300 tests
M041-0	GPIbalpha (CD42b)	Xia.H10	Rat IgG2a	purif.	FC, IHC	0.5 mg
M041-1	GPIbalpha (CD42b)	Xia.H10	Rat IgG2a	FITC	FC, IHC	1.5 ml; 300 tests
M042-0	GPIbalpha (CD42b)	Xia.G7	Rat IgG1	purif.	IP, IHC, WB	0.5 mg
M042-1	GPIbalpha (CD42b)	Xia.G7	Rat IgG1	FITC	FC, IHC	1.5 ml; 300 tests
M043-0	GPIbalpha (CD42b)	Xia.B2	Rat IgG2a	purif.	IP, IHC, block	0.5 mg
M043-1	GPIbalpha (CD42b)	Xia.B2	Rat IgG2a	FITC	FC, IHC	1.5 ml; 300 tests
M050-0	GPIbbeta (CD42c)	Xia.C3	Rat IgG2a	purif.	IP, IHC, WB	0.5 mg
M050-1	GPIbbeta (CD42c)	Xia.C3	Rat IgG2a	FITC	FC, IHC	1.5 ml; 300 tests
M051-0	GPIX (CD42a)	Xia.B4	Rat IgG1	purif.	IP, IHC	0.5 mg
M051-1	GPIX (CD42a)	Xia.B4	Rat IgG1	FITC	FC, IHC	1.5 ml; 300 tests
M060-1	GPV (CD42d)	Gon.C2	Rat IgG1	FITC	FC, IHC	1.5 ml; 300 tests
M061-1	GPV (CD42d)	Gon.G6	Rat IgG2a	FITC	FC, IHC	1.5 ml; 300 tests
M070-0	Integrin alpha2 chain (CD49b, GPIa)	Sam.G4	Rat IgG2b	purif.	IP, IHC, block	0.5 mg
M070-1	Integrin alpha2 chain (CD49b, GPIa)	Sam.G4	Rat IgG2b	FITC	FC, IHC	1.5 ml; 300 tests
M071-0	Integrin alpha2 chain (CD49b, GPIa)	Sam.C1	Rat IgG2b	purif.	IP, IHC	0.5 mg
M071-1	Integrin alpha2 chain (CD49b, GPIa)	Sam.C1	Rat IgG2b	FITC	FC, IHC	1.5 ml; 300 tests
M075-0	Integrin alpha2 chain (CD49b, GPIa)	Sam.C1, Sam.G4, Sam.H9	Rat IgG2b/IgG1	purif.	IP, IHC	0.5 mg
M080-0	Integrin alpha5 chain (CD49e)	Tap.A12	Rat IgG1	purif.	IP, IHC	0.5 mg
M080-1	Integrin alpha5 chain (CD49e)	Tap.A12	Rat IgG1	FITC	FC, IHC	1.5 ml; 300 tests
M110-0	CD9	Nyn.H3	Rat IgG2a	purif.	IP, IHC, WB	0.5 mg
M110-1	CD9	Nyn.H3	Rat IgG2a	FITC	FC, IHC	1.5 ml; 300 tests
M120-0	CD31 (PECAM-1)	Pec.H3	Rat IgG1	purif.	IP, IHC, WB	0.5 mg
M130-0	CD62P (P-selectin)	Wug.E9	Rat IgG1	purif.	IP, IHC	0.5 mg
M130-1	CD62P (P-selectin)	Wug.E9	Rat IgG1	FITC	FC, IHC	1.5 ml; 300 tests
M130-2	CD62P (P-selectin)	Wug.E9	Rat IgG1	PE	FC, IHC	1.5 ml; 300 tests
P140-1	fibrinogen	polyclonal	Rabbit IgG	FITC	FC, IHC	1.5 ml; 300 tests
P150-1	von Willebrand Factor (vWF)	polyclonal	Rabbit IgG	FITC	FC, IHC	1.5 ml; 300 tests
P180-1	negative control	polyclonal	Rabbit IgG	FITC	FC, IHC	1.0 ml; 200 tests
P190-1	negative control	polyclonal	Rat IgG	FITC	FC, IHC	1.0 ml; 200 tests
P190-2	negative control	polyclonal	Rat IgG	PE	FC, IHC	1.0 ml; 200 tests


P190-3	negative control	polyclonal	Rat IgG	DyLight 649	FC, IHC	1.0 ml; 200 tests
R300	Antibodies for Mouse Plaet Depletion	polyclonal anti-GPIb alpha	Rat IgG	purif.	plaet depletion	0.5 mg
X488	Antibodies for In Vivo Mouse Plaet Labeling	anti - GPIbbeta derivative	Rat IgG	DyLight 488	in vivo	100 µg
X649	Antibodies for In Vivo Mouse Plaet Labeling	anti - GPIbbeta derivative	Rat IgG	DyLight 649	in vivo	100 µg

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EMFRET R300 產(chǎn)品說明書