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當(dāng)前位置：首頁 > 技術(shù)文章 > α-凝血酶α-Thrombin選購指南

α-凝血酶α-Thrombin選購指南

更新時間：2016-11-01      點擊次數(shù)：4548

凝血酶Thrombin (Factor IIa)是一種絲氨酸蛋白酶，也是血液凝血級聯(lián)反應(yīng)中的主要效應(yīng)蛋白酶，顯現(xiàn)出促凝和抗凝的特性。凝血酶是由在凝血酶原復(fù)合物中的非活性凝血酶原在Xa因子（FXa）因子的作用下通過蛋白裂解的方式產(chǎn)生

【生理作用】當(dāng)循環(huán)凝血因子在暴露的血管外組織與組織因子相接觸時，凝血酶會在組織上聚集。凝血酶通過激活血小板，催化纖維蛋白原轉(zhuǎn)化為纖維蛋白，促進(jìn)血穩(wěn)定而在血栓性疾病的引發(fā)和傳播上有著核心作用

α-凝血酶（α-Thrombin）是一種高度特異性的絲氨酸蛋白酶，由凝血酶原（prothrombin，II因子）經(jīng)蛋白水解活化而來。它在凝血過程中起著非常重要的作用，不僅能夠促使纖維蛋白凝塊的產(chǎn)生，還負(fù)責(zé)提供反饋信號來激活前輔因子：V因子和VIII因子。也可以激活XIII因子和血小板，或者用作血管收縮劑。

在體內(nèi)，活化的X因子（Factor Xa）切割凝血酶原，釋放出活性肽并將凝血酶切割成具有催化活性的α-凝血酶。α-凝血酶由一條輕鏈（A chain）（Mw ~6,000）和一條重鏈（B chain）（Mw ~31,000）通過二硫鍵連接而成。某些情況下，α-凝血酶會發(fā)生自溶生成β-凝血酶和γ-凝血酶。β-凝血酶由對α-凝血酶A鏈水解并切割含有B鏈糖基化位點的小片段（B1，B2）組合而成。

除了用于凝血研究之外，α-凝血酶還常用來位點特異性切割融合蛋白?；蛑亟M時將凝血酶識別位點插入在目的蛋白與利于后續(xù)純化和/或表達(dá)的多肽或者蛋白之間，通過凝血酶切割表達(dá)的重組子即可釋放目的蛋白。凝血酶本身可通過親和層析技術(shù)快速去除。

作為生物活性物質(zhì)的凝血酶：是一種在人體抗凝，促凝的凝血級聯(lián)反應(yīng)中有著重要作用的生物活性蛋白。

產(chǎn)品性質(zhì)

中文別名（Chinese synonym）

α-凝血酶；IIa 因子；

英文別名（English synonym）

α-Thrombin; Factor IIa;

CAS號（CAS NO.）

9002-04-4

分子量（Molecular weight）

37000 daltons

活力（Activity）

≥2700.00 NIH U/mg

緩沖液組分（Buffer）

50mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5

消光系數(shù)（Extinction coefficient）（1%）

18.3

運輸和保存方法

粉末冰袋運輸，2-8℃保存。液體干冰運輸，≤-60℃保存。

凝血酶Thrombin (Factor IIa)  抗體

11571-05021Thrombin, anti-human150 ug2800AssayPro
11571-05011Thrombin, anti-human150 ug2320AssayPro
BP104CBovine anti-Thrombin (AT)1 x 10 mg4545hyphen biomed
60130-05021alpha-Thrombin (Factor IIa), anti-human150 ug2320AssayPro
60130-05011alpha-Thrombin (Factor IIa), anti-human150 ug2000AssayPro
SAHT-APAnti-human Thrombin (F.IIa) sheep, AP IgG,0.5 mg5168Enzyme Research2016
PAHT-SSheep anti-Human  Thrombin1x1 mg436.8Haematologic Technologies
PAHT-SSheep anti-Human  Thrombin1x5 mg1506.96Haematologic Technologies
SART-IGSheep anti  rabbit Thrombin  purified IgG10 mg2414affinitybiologicals
SART-HRPSheep anti  rabbit Thrombin  peroxidase conjugated IgG0.2 mg4131affinitybiologicals
SART-APSheep anti  rabbit Thrombin  affinity purified IgG0.5 mg5304affinitybiologicals
SAHT-IGSheep anti  human Thrombin  purified IgG10 mg2227affinitybiologicals
SAHT-HRPSheep anti  human Thrombin  peroxidase conjugated IgG0.2 mg4029affinitybiologicals
SAHT-APFTCSheep anti  human Thrombin  affinity purified, FITC conjugated IgG0.1 mg4029affinitybiologicals
SAHT-APBIOSheep anti  human Thrombin  affinity purified, Biotinylated IgG0.1 mg4029affinitybiologicals
SAHT-APSheep anti  human Thrombin  affinity purified IgG0.5 mg5168affinitybiologicals
SABT-IGSheep anti  bovine Thrombin  purified IgG10 mg2227affinitybiologicals
SABT-HRPSheep anti  bovine Thrombin  peroxidase conjugated IgG0.2 mg4029affinitybiologicals
SABT-APSheep anti  bovine Thrombin  affinity purified IgG0.5 mg5168affinitybiologicals
SART-IGAnti-rabbit Thrombin (rF.IIa)sheep, purified IgG,                                 10 mg10 mg2600Enzyme Research2016
SART-HRPAnti-rabbit Thrombin (rF.IIa)sheep, perox-conj. IgG,0.2 mg4131Enzyme Research2016
SART-APAnti-rabbit Thrombin (rF.IIa)sheep, affinity purified IgG,0.5 mg5304Enzyme Research2016
SATAFI-APAnti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, affinity purified IgG,0.5 mg5066Enzyme Research2016
SAHT-HRPAnti-human Thrombin (F.IIa)sheep, perox-conj. IgG,0.2 mg4029Enzyme Research2016
SAHT-IGAnti-human Thrombin (F.IIa) sheep, purified IgG,10 mg2600Enzyme Research2016
SAHT-APFTCAnti-human Thrombin (F.IIa) sheep, AP-FITC0.1 mg4029Enzyme Research2016
AHT-5020Anti-Human Thrombin1x100 µg1179.36Haematologic Technologies
AHT-5020Anti-Human Thrombin5x100 µg3057.6Haematologic Technologies
SABT-IGAnti-bovine Thrombin (bF.IIa)sheep, purified IgG,10 mg2600Enzyme Research2016
SABT-HRPAnti-bovine Thrombin (bF.IIa)sheep, perox-conj. IgG,0.2 mg4029Enzyme Research2016
SABT-APAnti-bovine Thrombin (bF.IIa)sheep, affinity purified IgG,0.5 mg5168Enzyme Research2016
SATAFI-IG Anti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, purified IgG,10 mg2635Enzyme Research2016

 

凝血酶Thrombin (Factor IIa)  試劑盒

貨號品名包裝價格品牌
ET4010-1Human Thrombin ELISA Kit96 well plate4760AssayPro
Thrombin-HCII ComplexThrombin-HCII Complex elisa kit5 plate8024Enzyme Research2016
ADG844OLIGOBIND® Thrombin activity assay96 microwell plate9200American Diagnostica Inc

凝血酶Thrombin (Factor IIa)  抑制劑

貨號品名包裝價格品牌
TAFI-01Thrombin-Activatable   Fibrinolysis Inhibitor1x50 µg4298.83272Haematologic Technologies
TAFI-01Thrombin-Activatable   Fibrinolysis Inhibitor1x1 mg68592.36384Haematologic Technologies
TAFI-01Thrombin-Activatable   Fibrinolysis Inhibitor5x50 µg19250.2674Haematologic Technologies
TAFI-01Thrombin-Activatable   Fibrinolysis Inhibitor5x1 mg308594.7774Haematologic Technologies
TAFI-01Thrombin-Activatable   Fibrinolysis Inhibitor10x1 mg555305.2596Haematologic Technologies
CK002LHEMOCLOT Thrombin Inhibitors3x2.5 mL2130hyphen biomed
CK002KHEMOCLOT Thrombin Inhibitors3x1 mL1110hyphen biomed

凝血酶Thrombin (Factor IIa)  發(fā)色底物

SN-17aD-FPR-ANSNH-C6H11 • 2 HCl100x1 mg184235.688Haematologic Technologies
SN-59D-VPR-ANSNH-C4H9 • 2 HCl1x1 mg1842.35688Haematologic Technologies
229005BIOPHEN CS-01(81) - Thrombin Chromogenic Substrate1 x 25 mg3000hyphen biomed
229001BIOPHEN CS-01(38) - Thrombin Chromogenic substrate1 x 25 mg3000hyphen biomed
0082032439S-2238   H-D-Phe-Pip-Arg-pNA•2HCl 25mg3500Chromogenix2014
SN-20Boc-L-FPR-ANSNH-C2H51x1 mg1842.35688Haematologic Technologies

凝血酶Thrombin (Factor IIa) ?其他耗材和試劑

ADG844T50Thrombin blood collection tubes, 3 ml50 tubes5525American Diagnostica Inc
ADG844T25Thrombin blood collection tubes, 3 ml25 tubes3025American Diagnostica Inc
CK011LHEMOCLOT Thrombin Time (T.T.)6 x 8 mL915hyphen biomed
CK011KHEMOCLOT Thrombin Time (T.T.)6 x 2 mL285hyphen biomed
CK001LHEMOCLOT Thrombin (human)6 x 8 mL1155hyphen biomed
CK001KHEMOCLOT Thrombin (human)6 x 2 mL420hyphen biomed

凝血酶Thrombin (Factor IIa)  蛋白

貨號品名包裝價格品牌
PT62082Thrombin, Mouse50 ug3900AssayPro
PP004KAnti-Thrombin (AT)6 x 150 µg3030hyphen biomed
PP004BAnti-Thrombin (AT)1 x 1.5 mg1545hyphen biomed
PP004AAnti-Thrombin (AT)1 x 150 µg720hyphen biomed
HT 1002aHuman a-Thrombin Priced per 1000 units3,000+ units2600Enzyme Research2016
HT 1002aHuman a-Thrombin Priced per 1000 units<3,000 units2600Enzyme Research2016
BT 1002aBovine Thrombin (Priced per 1000 units)<3,000  units    2600Enzyme Research2016
RIIaRat Thrombin0.1 mg5199.9192Enzyme Research2016
RbIIaRabbit Thrombin0.1 mg5199.9192Enzyme Research2016
PIIaPorcine Thrombin0.1 mg5199.9192Enzyme Research2016
MIIaMurine Thrombin0.05 mg2600Enzyme Research2016
MCT-5020Mouse Thrombin1x50 µg4004.1456Haematologic Technologies
MCT-5020Mouse Thrombin5x50 µg17771.208Haematologic Technologies
MCT-5020Mouse Thrombin1x1 mg63116.34Haematologic Technologies
MCT-5020Mouse Thrombin25x1 mg1277277.456Haematologic Technologies
MCT-5020Mouse Thrombin5x1 mg283889.424Haematologic Technologies
HTi  Human Thrombin Inactivated1.0 mg2600Enzyme Research2016
EZ006OHuman Thrombin1 x 10 NIH195hyphen biomed
EZ006KHuman Thrombin6 x 10 NIH825hyphen biomed
EZ006BHuman Thrombin1 x 1000 NIH3765hyphen biomed
HCGT-0021Human gamma-thrombin1x100 µg992.03832Haematologic Technologies
HCGT-0021Human gamma-thrombin5x100 µg4487.7924Haematologic Technologies
HCGT-0021Human gamma-thrombin5x1 mg38736.7344Haematologic Technologies
HCGT-0021Human gamma-thrombin10x1 mg76292.4708Haematologic Technologies
HCGT-0021Human gamma-thrombin50x1 mg343670.418Haematologic Technologies
HCGT-0021Human gamma-thrombin100x1 mg670806.864Haematologic Technologies
HCGT-0021Human gamma-thrombin500x1 mg3035164.86Haematologic Technologies
HCBT-0022Human beta-thrombin1x100 µg992.03832Haematologic Technologies
HCBT-0022Human beta-thrombin5x100 µg4487.7924Haematologic Technologies
HCBT-0022Human beta-thrombin5x1 mg38736.7344Haematologic Technologies
HCBT-0022Human beta-thrombin10x1 mg76292.4708Haematologic Technologies
HCBT-0022Human beta-thrombin50x1 mg343670.418Haematologic Technologies
HCBT-0022Human beta-thrombin100x1 mg670806.864Haematologic Technologies
HCBT-0022Human beta-thrombin500x1 mg3035164.86Haematologic Technologies
HCT-FPRCKHuman alpha-thrombin-FPRCK1x100 µg850.31856Haematologic Technologies
HCT-FPRCKHuman alpha-thrombin-FPRCK1x1mg5172.77124Haematologic Technologies
HCT-FPRCKHuman alpha-thrombin-FPRCK5x100 µg3542.994Haematologic Technologies
HCT-FPRCKHuman alpha-thrombin-FPRCK5x1mg25509.5568Haematologic Technologies
HCT-FPRCKHuman alpha-thrombin-FPRCK10x1mg46295.1216Haematologic Technologies
HCT-FPRCKHuman alpha-thrombin-FPRCK50x1mg226751.616Haematologic Technologies
HCT-FPRCKHuman alpha-thrombin-FPRCK100x1mg403901.316Haematologic Technologies
HCT-DFPHuman alpha-thrombin-DFP1x100 µg850.31856Haematologic Technologies
HCT-DFPHuman alpha-thrombin-DFP1x1 mg5172.77124Haematologic Technologies
HCT-DFPHuman alpha-thrombin-DFP5x100 µg3542.994Haematologic Technologies
HCT-DFPHuman alpha-thrombin-DFP5x1 mg25509.5568Haematologic Technologies
HCT-DFPHuman alpha-thrombin-DFP10x1 mg46295.1216Haematologic Technologies
HCT-DFPHuman alpha-thrombin-DFP50x1 mg226751.616Haematologic Technologies
HCT-DFPHuman alpha-thrombin-DFP100x1 mg403901.316Haematologic Technologies
HCT-BFPRCKHuman alpha-thrombin-BFPRCK1x100 µg850.31856Haematologic Technologies
HCT-BFPRCKHuman alpha-thrombin-BFPRCK1x1 mg5172.77124Haematologic Technologies
HCT-BFPRCKHuman alpha-thrombin-BFPRCK5x100 µg3542.994Haematologic Technologies
HCT-BFPRCKHuman alpha-thrombin-BFPRCK5x1 mg25509.5568Haematologic Technologies
HCT-BFPRCKHuman alpha-thrombin-BFPRCK10x1 mg46295.1216Haematologic Technologies
HCT-BFPRCKHuman alpha-thrombin-BFPRCK50x1 mg226751.616Haematologic Technologies
HCT-BFPRCKHuman alpha-thrombin-BFPRCK100x1 mg403901.316Haematologic Technologies
HCT-0020Human alpha-thrombin1x100 µg698.88Haematologic Technologies
HCT-0020Human alpha-thrombin1x1 mg2140.32Haematologic Technologies
HCT-0020Human alpha-thrombin5x100 µg2948.4Haematologic Technologies
HCT-0020Human alpha-thrombin5x1 mg9172.8Haematologic Technologies
HCT-0020Human alpha-thrombin10x1 mg16816.8Haematologic Technologies
HCT-0020Human alpha-thrombin50x1 mg81900Haematologic Technologies
HCT-0020Human alpha-thrombin100x1 mg146328Haematologic Technologies
HCT-0020Human alpha-thrombin500x1 mg677040Haematologic Technologies
HGTH. Gamma Thrombin1.0 mg6827.1048Enzyme Research2016
CIIaCanine Thrombin0.1 mg5199.9192Enzyme Research2016
BT 1002aBovine Thrombin (Priced per 1000 units)3,000+ units2600Enzyme Research2016
BE501KBovine Thrombin10 x 500 NIH14850hyphen biomed
BE501CBovine Thrombin1 x 500 NIH1875hyphen biomed
BE102KBovine Thrombin6 x 21 NIH885hyphen biomed
BE102CBovine Thrombin1 x 1000 NIH3030hyphen biomed
BE102BBovine Thrombin1 x 100 NIH735hyphen biomed
BE102ABovine Thrombin1 x 21 NIH195hyphen biomed
BCT-FPRCKBovine alpha-thrombin-FPRCK1x200 µg992.03832Haematologic Technologies
BCT-FPRCKBovine alpha-thrombin-FPRCK1x1 mg3377.65428Haematologic Technologies
BCT-FPRCKBovine alpha-thrombin-FPRCK5x200 µg4015.3932Haematologic Technologies
BCT-FPRCKBovine alpha-thrombin-FPRCK5x1 mg16652.0718Haematologic Technologies
BCT-FPRCKBovine alpha-thrombin-FPRCK10x1 mg29761.1496Haematologic Technologies
BCT-FPRCKBovine alpha-thrombin-FPRCK50x1 mg145262.754Haematologic Technologies
BCT-FPRCKBovine alpha-thrombin-FPRCK100x1 mg266905.548Haematologic Technologies
BCT-DFPBovine alpha-thrombin-DFP1x200 µg992.03832Haematologic Technologies
BCT-DFPBovine alpha-thrombin-DFP1x1 mg3377.65428Haematologic Technologies
BCT-DFPBovine alpha-thrombin-DFP5x200 µg4015.3932Haematologic Technologies
BCT-DFPBovine alpha-thrombin-DFP5x1 mg16652.0718Haematologic Technologies
BCT-DFPBovine alpha-thrombin-DFP10x1 mg29761.1496Haematologic Technologies
BCT-DFPBovine alpha-thrombin-DFP50x1 mg145262.754Haematologic Technologies
BCT-DFPBovine alpha-thrombin-DFP100x1 mg266905.548Haematologic Technologies
BCT-BFPRCKBovine alpha-thrombin-BFPRCK1x200 µg992.03832Haematologic Technologies
BCT-BFPRCKBovine alpha-thrombin-BFPRCK1x1 mg3377.65428Haematologic Technologies
BCT-BFPRCKBovine alpha-thrombin-BFPRCK5x200 µg4015.3932Haematologic Technologies
BCT-BFPRCKBovine alpha-thrombin-BFPRCK5x1 mg16652.0718Haematologic Technologies
BCT-BFPRCKBovine alpha-thrombin-BFPRCK10x1 mg29761.1496Haematologic Technologies
BCT-BFPRCKBovine alpha-thrombin-BFPRCK50x1 mg145262.754Haematologic Technologies
BCT-BFPRCKBovine alpha-thrombin-BFPRCK100x1 mg266905.548Haematologic Technologies
BCT-1020Bovine alpha-thrombin1x200 µg786.24Haematologic Technologies
BCT-1020Bovine alpha-thrombin1x1 mg2140.32Haematologic Technologies
BCT-1020Bovine alpha-thrombin5x200 µg3494.4Haematologic Technologies
BCT-1020Bovine alpha-thrombin5x1 mg9172.8Haematologic Technologies
BCT-1020Bovine alpha-thrombin10x1 mg16816.8Haematologic Technologies
BCT-1020Bovine alpha-thrombin50x1 mg81900Haematologic Technologies
BCT-1020Bovine alpha-thrombin100x1 mg146328Haematologic Technologies
BCT-1020Bovine alpha-thrombin500x1 mg677040Haematologic Technologies
0081079639AT III 1x25IU1x25IU5500Chromogenix2014
0082072039AT III 10x10 IU10x10 IU14780Chromogenix2014
470HTalpha-thrombin, human2,500 unit5628

American Diagnostica Inc

 

 

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